How do you preserve stick insects?

How do you preserve stick insects?

Store the leaves in a jar of water; this will keep them fresh for longer. Care should be taken that the stick insects can’t fall into the water container and drown. Stick insects get all their food and water requirements from the leaves that they eat.

What do aquatic invertebrates eat?

As for diet, aquatic invertebrates are a type of heterotroph known as omnivores; they eat plants, other insects, and, in some cases, small fish. Most aquatic insects, such as dragonflies or damselflies, begin their lives living directly in the water.

What is EPT metric?

The EPT Index is the total number of distinct taxa within the groups, Trichoptera, Ephemeroptera, and Plecoptera. For example, if five species of Ephemeroptera (mayflies), five Plecoptera (stoneflies), and two Trichoptera (caddisflies) are found at a site, the total number of EPT taxa and Index would equal 12.

How do you dry moths?

I like to place a small piece of mesh screen underneath so that it does not sit directly on the paper towel. Add another damp paper towel on top (domed over so that it does not sit directly on the specimen). Close the lid tightly and leave undisturbed for 24-48 hours.

How do you catch invertebrates?

Invertebrates on trees and bushes

  1. Pooter. A pooter is a flexible tube that can be used to catch invertebrates directly from leaves.
  2. Kite nets and sweep nets. Kite nets are lightweight nets used for catching insects flying above the vegetation layer.
  3. Beating tray.

What does EPT stand for in biology?

In upstream rivers, insects from the order Ephemeroptera, Plecoptera and Trichoptera (EPT) usually occur in large numbers (Al-Shami et al.

How do you do kick sampling?

Kick sampling Use one foot to kick the bottom of the stream, dislodging the substrate in the direction of the net. Animals dislodged from the substrate will be washed into the net. As sampling disturbs the substrate, always take the first sample at the lowest point upstream, then work back upstream.

What is the pollution tolerance index rating?

The Pollution Tolerance Index (PTI) is a comparison of the abundance of taxa and their tolerance to environmental stress. The index can indicate organic and nutrient pollution. The higher the PTI, the better the water quality.

What is the BMWP biotic index?

The biological monitoring working party (BMWP) is a procedure for measuring water quality using families of macroinvertebrates as biological indicators. The BMWP score equals the sum of the tolerance scores of all macroinvertebrate families in the sample.

How do you calculate biotic index?

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  1. Example of Biotic Index Calculation. Using Macroinvertebrate Information.
  2. If you find:
  3. Mayflies. Caddisflies. Stoneflies. Scuds. Midge larva.
  4. Multiply each by the Biotic Value:
  5. Biotic Value. Mayflies. 25 x 10 = 250. Caddisflies. 15 x 10 = 150.
  6. TOTAL = 820. Divide total by 10. This is the Biotic Index Value: 820/10 = 82.

What is a good biotic index?

Biotic indices based on the classification of species into groups of tolerance and/or associated to diversity have been used to track anthropogenic change….Data Analyses.

Family Biotic Index Water Quality
0.00–3.75 Excellent
3.76–4.25 Very good
4.26–5.00 Good
5.01–5.75 Fair

How do you collect aquatic invertebrates?

Put some fresh water into your smaller containers and begin sorting the invertebrates by appearance. You can pick them up gently with the forceps or use a plastic spoon or small container to scoop them.

How do you pin a bug?

Pick up the small insect carefully with forceps and mount it by touching it on its thorax (right side) to the drop of glue. Adjust the insect so that it remains squarely in position, then allow the glue to dry. You can also use nail polish instead of glue. Pins # 2 or 3 should be used.

Do butterflies feel pain?

Butterflies do not feel pain. Although butterflies know when they are touched, their nervous system does not have pain receptors that registers pain so this procedure did not cause the butterfly stress or pain.

How do you sample macroinvertebrates?

Sampling Methods: Sample with a kick net by holding the net frame firmly against the stream bottom and disturbing the substrate upstream (approximately a full arm’s length) from the net with your feet. Dig deeply into the substrate with the heel or toe to dislodge macroinvertebrates from the streambed.

Why are EPT taxa useful as bioindicators?

EPT are very much intolerable to any presence of pollutants in the water bodies and thus EPT are crucial biological indicators in determining water quality of the river.

How do you kill and preserve insects?


  1. Freeze them. Place collected insects inside small Tupperware containers or plastic bags and put them in the freezer for a few hours to kill them.
  2. Use a kill jar. This is a special glass jar with Plaster of Paris on the bottom that you saturate with nail polish remover or some other poison.

Why are insects dry before storage?

The purpose of storage is to provide the dried grain with protection against insects, molds, rodents and birds, and to prevent moisture from re-entering the grain. BUT if the drying is not done properly, you will have losses no matter how good the storage is.

What kind of insects can be collected using aquatic net?

Dragonflies and Damselflies Odonates are relatively easy to collect with an aquatic D-net for nymphs or a sweep net for adults.

How do you show a moth?

After a butterfly or moth is relaxed, carefully hold the specimen under its thorax between your thumb and forefinger. Gently squeeze the thorax; the wings should separate slightly. 2. From the top, insert a pin through the center of the thorax.

What Is percent EPT?

Percent EPT is the total number of EPT individuals divided by the total number of individuals in the sample. The HBI is based on an organism’s relative sensitivity to stream-quality conditions.

How is HBI calculated?

The equation is HBI = sum of [(xi x ti) / n] where xi = number of individuals within a taxon; ti = tolerance value of that taxon and n = total number of organisms in the sample with a tolerance value. HBI is the average of tolerance value of the sample.

Are fish macroinvertebrates?

In wadable streams (streams that can be easily walked across, with water no deeper than about thigh-high), the three most common biological organisms studied are fish, algae, and macroinvertebrates. They inhabit all types of running waters, from fast-flowing mountain streams to slow-moving muddy rivers.

How is BMWP score calculated?

The BMWP index is calculated by adding up the individual tolerance scores of aquatic macroinvertebrates at family taxonomic level present at a sample site.

Where do you pin a cockroach?

Pin through the centre of the thorax between the forewings, a little to the right of the centre. The front edges of the wings are placed at 45° to the body. Pin through the centre of the thorax with wings displayed at both sides of the body; overlap of fore and hind wings at 90° to the body.

What does a high biotic index mean?

From Wikipedia, the free encyclopedia. Biotic Index (also called “Family Biotic Index”) is a scale for showing the quality of an environment by indicating the types of organisms present in it. It is often used to assess the quality of water in rivers.

How do you preserve a dead moth?

Fill a small glass jar halfway full with rubbing alcohol. The rubbing alcohol will preserve the body of the insect and prevent it from decaying, drying out, or breaking into pieces. The jar should be larger than the insect, but not unnecessarily so.

What is a Surber sampler?

Surber samplers are used for taking quantitative samples of organisms that live in the sediment or gravel of stream beds where the water flow is less than 10cm/s.

What do you do when you find a dead butterfly?

If your butterfly is freshly dead, but you can’t get to it right away, put it in a glassine envelope and pop it in the freezer for a few days until you can get to it. The freezer will keep it fresh. But, I wouldn’t leave it in there too long, for more than a couple weeks because it will eventually dry out.