Which antibiotic resistance gene is present in pBR322?

The plasmid pBR322 vector carries the genes for tetracycline (tetR) and ampicillin (ampR) resistance. These genes are useful to identify and select the transformants and non-transformants.

Which sequence of pBR322 is responsible for?

So, the correct answer is ‘Ori site’

What is the ColE1 origin?

ColE1 is a plasmid found in bacteria. Its name derives from the fact that it carries a gene for colicin E1 (the cea gene). It also codes for immunity from this product with the imm gene. In addition, the plasmid has a series of mobility (mob) genes.

What is the role of tetracycline resistance gene in pBR322?

Plasmid pBR322 and its numerous derivatives are used extensively for research and in biotechnology. The tetracycline-resistance (TcR) genes in these plasmids are expressed constitutively and cells carrying these plasmids are resistant to tetracycline.

Who discovered plasmid pBR322?

This part of the vector name stands for Bolivar and Rodriguez, two of the scientists who constructed the pBR322 cloning vector in 1977. They named the plasmid with the number “322” to distinguish this vector from other vectors they developed in their laboratory.

What is pBR in pBR322?

In plasmid pBR 322, ‘BR’ stands for Bolivar and Rodriguez, the scientists who constructed the plasmid. The letter ‘p’ stands for the plasmid.

Who discovered pBR322?

pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolivar Zapata, the postdoctoral researcher and Raymond L. Rodriguez.

What is F1 origin?

The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive within cells (Addgene). F1 is a phage-derived ori that allows for the replication and packaging of ssDNA into phage particles. Plasmids with phage-derived ori’s are referred to as phagemids.

What is the sequence of origin of replication?

An origin of replication is a sequence of DNA at which replication is initiated on a chromosome, plasmid or virus. For small DNAs, including bacterial plasmids and small viruses, a single origin is sufficient.

What is tetracycline resistance gene?

The tet(W) tetracycline resistance gene encodes a protection protein that attaches to the ribosome and causes an alteration of the ribosomal conformation to which tetracycline cannot bind and therefore protein synthesis can proceed (Chopra and Roberts, 2001; Connell et al., 2003).

Where is pBR322 derived?

pBR322 is isolated from E. coli ER2686 (dam +dcm + EcoK M-) by a standard plasmid purification procedure.

Which statement is true for pBR322?

Which of the statement is true for pBR322? Explanation: pBR 322 is the man-made vector. It contains both ampicillin resistant and tetracycline resistant genes. The cloning site is also present in both of the genes.

What is the structure of pBR322?

pBR322 is 4361 base pairs in length and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (Amp R) protein, and the gene tetA encoding the tetracycline resistance (Tet R) protein. It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number.

Are the antibiotic-resistant genes on pBR322 transposable?

The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI.

What is plasmid pBR322 used for?

Plasmid pBR322 is used extensively in genetic engineering. It has two genes of special interest. One codes for a protein that enables any host bacterium to resist the lethal effects of the antibiotic ampicillin and the other confers resistance to tetracycline. The latter contains a sequence of six bases recognised by the restriction enzyme Bam HI.

What is the history of E coli plasmid pBR322?

pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco , it was named after Francisco Bolivar Zapata , the postdoctoral researcher who constructed it.