What is anti histidine?

Anti-histidine antibodies can be used to monitor the progress of purification and the fraction containing the recombinant protein by western blotting, and to verify that no proteolytic degradation of the recombinant protein is taking place.

What is 6x His-tag?

The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells.

How does his-tag purification work?

His-tag purification uses the purification technique of immobilized metal affinity chromatography, or IMAC. In this technique, transition metal ions are immobilized on a resin matrix using a chelating agent such as iminodiacetic acid.

Is histidine an antibody?

Both polyclonal and monoclonal antibodies against the polyhistidine tag are available commercially. The small polyhistidine tag with six histidine residues is not immunogenic; therefore a carrier protein is conjugated to the tag to elicit an immune response in a host animal….Figure 2.

What is his tag antibody?

His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with a protein of unknown origin ~60-70kDa.

What is an N terminal His-tag?

A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

What is a His-tag used for?

One of the most commonly used tags is the polyhistidine tag, also known as His-Tag, which is a string of usually between six and nine histidine residues (see Figure 1 below). This method of tagging is especially useful as it allows for easy purification and detection of the recombinant protein.

Is His-tag fluorescent?

The probe, driven by Ni(2+)-NTA, binds specifically to His-tags genetically fused to proteins and subsequently forms a covalent bond upon photoactivation of the arylazide, leading to a 13-fold fluorescence enhancement.

Why is His-tag used?

What are his tags used for?

The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. These residues readily coordinate with transition metal ions such as Ni2+ or Co2+ immobilized on beads or a resin for purification.

What is a His-tag antibody?

His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

What is anti-6x his tag® antibody?

Overview Product name Anti-6X His tag® antibody [HIS.H8] Description Mouse monoclonal [HIS.H8] to 6X His tag® Host species Mouse Specificity Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. Tested applications Suitable for: ICC/IF, ELISA, Dot blot, WB, IP, EMSAmore details

What is a 6x His tag?

Target Information. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography.

What is the use of his fusion antibody?

This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells. This product contains enough material for 25 Western blots.

What antibody is used in western blot?

Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.