What is a GT B fold?

The GT-B fold is comprised of two distinct N-terminal and C-terminal Rossmann-like domains of six or seven parallel β-sheet linked to α-helices, connected by a linker region and a interdomain cleft [1] (Figures 1b and 1d).

Is UDP a glycosyltransferase?

UDP-glycosyltransferases (UGTs) are important conjugation enzymes found in all kingdoms of life, catalyzing a sugar conjugation with small lipophilic compounds and playing a crucial role in detoxification and homeostasis.

Where are glycosyltransferases located?

the Golgi apparatus
Glycosyltransferases (GT) catalyze the transfer of carbohydrate from nucleotide sugar substrates to incomplete glycolipid or glycoprotein acceptors. Although they are predominantly located in the Golgi apparatus, they have been reported to occur on the surface of a variety of cells, including lymphocytes (1).

Is glycan a carbohydrate?

Glycans, also called polysaccharides, are carbohydrate-based polymers made by all living organisms.

Does kinase use water?

The correct answer is a kinase. Kinases are enzymes that couple the hydrolysis of ATP to the addition of a phosphate group to its substrate. Phosphatase enzymes basically function oppositely to how kinases work. Phosphatases use water to hydrolyze phosphate groups off of their substrate.

What is the 3d-glycosyltransferase database?

To accommodate the growing number of crystal structures, we created the 3D-Glycosyltransferase database to gather structural information concerning this class of enzymes.

What is a glycosyl transfer enzyme?

Glycosyltransferases (GTs; EC 2.4.x.y) constitute a large family of enzymes that are involved in the biosynthesis of oligosaccharides, polysaccharides, and glycoconjugates ( Taniguchi et al ., 2002 ). These molecules of enormous diversity mediate a wide range of functions from structure and storage to signalling.

Do glycosyltransferase mechanisms have flexible loops?

Since both inverting and retaining glycosyltransferases undergo large conformational movements (Figure 4 ), the flexible loops that are proposed in GT mechanisms have been the subject of pioneering studies by molecular dynamic simulation.

What is bovine α1 3 galactosyltransferase catalytic domain?

) Bovine α1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases. –649. ) Crystal structure of the autocatalytic initiator of glycogen biosynthesis, glycogenin.