Can Qubit distinguish between RNA and DNA?

It’s important to note that fluorescence-based quantification using the Qubit system distinguishes between RNA and DNA in the same sample whereas UV absorbance readings typically do not.

How does Qubit bind to DNA?

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA).

What does a qubit do?

Quantum computers aren’t limited to two states; they encode information as quantum bits, or qubits, which can exist in superposition. Qubits represent atoms, ions, photons or electrons and their respective control devices that are working together to act as computer memory and a processor.

Are Nanodrops accurate?

Actually nanodrop is not a quite accurate method especially when trying to measure plasmid concentration. Your readings look like oscillating around -400 ng/ul, which I would take.

What does a Qubit do?

What is Qubit DNA?

Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance. More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA. Uses as little as 1 μL of sample, even with very dilute samples.

What dye does Qubit use?

fluorescent dyes
The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.

How accurate is the qubit dsDNA BR assay?

The Qubit dsDNA BR assay is intended to be accurate for initial sample concentrations ranging from 0.2 ng/µL to 1000 ng/µL. Both kits provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards.

What is the range of qubit RNA XR?

Qubit RNA XR Assay Kit – Extended Range The assays are accurate for initial sample concentrations from as little as 250 pg/μL to 10,000 ng/μL. These kits are highly selective for RNA over double-stranded DNA (dsDNA). Unlike other RNA assays, they do not require DNase if DNA is present in the sample for an accurate measurement.

Can I use the qubit flex fluorometer with my qubit dsDNA assay?

The Qubit 4 and Qubit Flex Fluorometer and all Qubit assays are optimized for use together. Our Qubit dsDNA high-sensitivity (HS) and broad-range (BR) assays are highly selective for double-stranded DNA (dsDNA) over RNA and single-stranded DNA (ssDNA).

What is a qubit assay?

Sensitive and specific nucleic acid quantification assays. The Qubit assays utilize target-selective dyes that emit fluorescence when bound to DNA, RNA or protein. Unlike UV absorbance, which can overestimate sample concentrations due to contaminants in the sample such as salts, solvents, detergents, proteins, free nucleotides.