What is jetPEI?

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells.

What is PEI transfection?

The polycation polyethylenimine (PEI) is one of the most utilized reagents for small- to large-scale transfections as it is simple to use and, when combined with optimized expression vectors and cell lines, provides high transfection efficiency and titers.

What is jetPRIME?

jetPRIME® is a proprietary cationic polymer-based molecule, synthesized at Polyplus-transfection®. jetPRIME® forms positively charged complexes with plasmid DNA, which penetrate the cell through endocytosis. Plasmid DNA is then released from the endosomes in the cytosol via the proton sponge mechanism.

What is PEI pro?

PEIpro® product range (PEIpro®, PEIpro®-HQ and PEIpro®-GMP) is the transfection method of choice to achieve reliable viral vector production and high infectious titer yields, with direct scalability and seamless transition from process development up to large-scale clinical-grade manufacturing.

What is a transfection reagent?

Transfection reagents are used to introduce naked or purified nucleic acids into eukaryotic cells. These products may include plasmids, reagents, kits, primer sets, and more.

Why is PEI used in transfection?

Conversely, PEI is widely used due to its affordability, high DNA delivery efficiency, and reproducibility during upscaling in both adherent and suspension cells, unlike calcium phosphate that is inefficient for transfection of cells grown in suspension.

How does PEI work?

PEI condenses DNA into positively charged particles, which bind to anionic cell surface residues and are brought into the cell via endocytosis. Once inside the cell, protonation of the amines results in an influx of counter-ions and a lowering of the osmotic potential.

What is the difference between lipofectamine 2000 and 3000?

Lipofectamine 3000 reagent yields higher transfection efficiencies than Lipofectamine 2000 reagent when tested in a variety of cell lines.

What is the jetPEI ® reverse transfection protocol?

Fig. 1: jetPEI ® reverse transfection protocol for HTS application. Complexes formed with the water-soluble polymer jetPEI ® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).

How is jetPEI ® tested?

Every batch of jetPEI ® is tested in-house by DNA transfection of HeLa cells. Transfection with a firefly Luciferase gene under the control of CMV promoter gives at least 10 9 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

What types of cells can jetPEI ® be used on?

jetPEI ® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI ® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.

Is jetPEI ® reagent suitable for plasmid DNA transfection?

Our jetPEI ® reagent is perfectly well suited for plasmid DNA transfection, especially for High-Troughput Screening (HTS) application. Read more… Every batch of jetPEI ® is tested in-house by DNA transfection of HeLa cells.