What technology is used for genome sequencing?

What is NGS? Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA.

When was pyrosequencing invented?

The principle of pyrosequencing was first described in 1993 by, Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing method using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the …

What is sequencing technique?

DNA sequencing refers to the general laboratory technique for determining the exact sequence of nucleotides, or bases, in a DNA molecule. The sequence of the bases (often referred to by the first letters of their chemical names: A, T, C, and G) encodes the biological information that cells use to develop and operate.

Is PCR a DNA sequencing technique?

Using PCR, a DNA sequence can be amplified millions or billions of times, producing enough DNA copies to be analyzed using other techniques. For instance, the DNA may be visualized by gel electrophoresis, sent for sequencing, or digested with restriction enzymes and cloned into a plasmid.

Is Roche 454 still used?

In late March 2007, Roche Diagnostics acquired 454 Life Sciences for US$154.9 million. It remained a separate business unit. In October 2013, Roche announced that it would shut down 454, and stop supporting the platform by mid-2016.

What is post-light sequencing?

Ion Torrent (another Life Technologies product) is the first so-called ‘post-light sequencing’ technology, as it uses neither fluorescence nor luminescence [69].

What was the last second-generation sequencing platform?

The last remarkable second-generation sequencing platform is that developed by Jonathan Rothburg after leaving 454. Ion Torrent (another Life Technologies product) is the first so-called ‘post-light sequencing’ technology, as it uses neither fluorescence nor luminescence [69].

What is the first-generation DNA sequencing technology?

This was the first technique to be widely adopted, and thus might be considered the real birth of ‘first-generation’ DNA sequencing. Open in a separate window Fig. 1 First-generation DNA sequencing technologies. Example DNA to be sequenced (a) is illustrated undergoing either Sanger (b) or Maxam–Gilbert (c) sequencing.

What was the first high-throughput sequencing (HTS) machine?

The first high-throughput sequencing (HTS) machine widely available to consumers was the original 454 machine, called the GS 20, which was later superceded by the 454 GS FLX, which offered a greater number of reads (by having more wells in the ‘picotiter’ plate) as well as better quality data [56].