What is MaxiSorp?

MaxiSorp and PolySorp surfaces were developed for immunology assays. The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity to molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation.

What is Nunc plate?

Thermo Scientific™ Nunc MicroWell 96-Well Microplates are optimized for robotics, automated readers and liquid handling systems. 96-well microplates are used for antibiotic screens, cell-based assays and screening compounds. The flat bottom shape is ideal for microscopic and optical measurements.

What are MaxiSorp plates?

The Nunc MaxiSorp™ high protein-binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready-SET-Go! ELISA kits, as well as other matched antibody pairs for ELISA. Maxisorp plates have a protein-binding capacity of approximately 600-650 ng IgG/cm2.

What is Elisa protocol?

Detailed Protocol. Sandwich ELISA is based on the detection and quantification of target protein (antigen), which is sandwiched between primary and secondary antibodies, each binding to a different epitope of the target antigen. This is a general protocol for sandwich ELISA.

What is Elisa plate?

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.

What is the purpose of a well plate?

noun. A rectangular plate or block containing multiple small wells or cavities, used for analysis, clinical testing, or culture; specifically = microtiter plate .

How do you coat plate on Elisa?

Coating of Capture Antibody

1. Appropriately dilute the capture or coating antibody in carbonate-bicarbonate buffer or PBS.
2. Pipette 0.2 ml of the diluted capture antibody to each well of a microtiter plate.
3. Incubate the plate (covered) for 1 hour at 37 °C.
4. Remove the coating solution.

What is coating buffer in Elisa?

Coating buffers stabilize the antigen or antibody which is used to coat the ELISA multiwell plate, maximizing adsorption to the plate and optimizing interactions with the detection antibody.

How do I set up an Elisa assay?

Steps to run a sandwich ELISA assay

1. Step 1: Capture antibody binds to ELISA plate wells.
2. Step 2: Add sample to well – antigen within the sample binds to the capture antibody.
3. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest.

How long do ELISA assays take?

Depending on what the test is being used for, you may get results as quickly as about 24 hours if the test is done locally. However, there are some tests that may take days to weeks.

How do I set up an ELISA?

What is the protein binding capacity of Nunc maxisorp plates?

Description The Nunc MaxiSorp™ high protein-binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready-SET-Go! ELISA kits, as well as other matched antibody pairs for ELISA. Maxisorp plates have a protein-binding capacity of approximately 600-650 ng IgG/cm 2.

What is the maxisorp surface?

The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity to molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation.

Why Thermo Scientific™ Nunc fluoronunc 96-well solid immuno plates?

Use Thermo Scientific™ Nunc FluoroNunc/LumiNunc 96-Well Solid plates for quantitative and qualitative solid phase immunoassays (e.g., FLISA) and binding assays. Thermo Scientific Nunc Immuno plates offer lot-to-lot reliability, well-to-well reproducibility, and are backed by industry-leading quality standards and certifications.

What is the maximum binding capacity of maxisorp and polysorp?

Using IgG as a reference molecule and knowing that it is a globular molecule, theoretical calculations indicate that the maximum binding for the MaxiSorp surface, in monolayer, is 650 ng/cm 2. For the PolySorp surface, the binding capacity is 220 ng/cm 2.